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TUNEL was performed on colonic tissue slices by applying the specific reaction reagents containing terminal deoxynucleotidyl transferase and fluorescein-labeled dUTP for a 1-h incubation period at 37 C
The funder had no role in the design, data collection, data analysis, and reporting of this study
No representations are made regarding biological activity, physiological outcomes, or experimental effects
Overuse of stimulants : Excessive use of stimulants like caffeine can exacerbate HPA axis dysfunction, while smoking and alcohol can worsen inflammation, interfere with sleep, and have other negative effects