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Slides were washed in PBS and incubated for 2 h in a mixture of PBS supplemented with 0.2% Triton X-100 and 0.1% bovine serum albumin, followed by incubation overnight with the primary antibodies (listed in Additional file 2: Table S2)
Risks: Allergic reactions, infections, or blocked blood vessels which can cause blindness or stroke
Its brief half-life (about 30 minutes) avoids constant stimulation by imitating physiological GHRH pulses
This suggests that although stress intensity enhances RIDD to favor cell death, this IRE1 activity may have other functions (Iwakoshi et al
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