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However, in this case, cortisol values are accumulated over a longer period so solutions need to be developed to take this problem into account

This observation contrasts with other normal and malignant proliferative cells such as intestinal and cancer cells that display reduced proliferation upon G6PDH inactivation 26

However, its important to recognize that a myopic policy isnt always the best choice when S tu values are high

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Especially since the enzymatic assays are costly, laborious and only performed in a limited number of centers

Each reaction mixture was prepared using 10 l QuantiFast SYBR Green PCR master mix, 4 l of cDNA template with 1 M of each primer in a total reaction volume of 20 l