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Internalised receptor from GLP-1-treated cells was quantified from cell-containing regions as determined from the phase contrast images using PHANTAST (13) and used to determine receptor internalisation as previously described (12)

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Quality control: Instrument calibration and quality control products (Level 1 & 2, Roche Diagnostics) were performed daily, with an intra-batch coefficient of variation (CV) of less than 5%

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