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Reduced L-glutathion is: the bioactive form of L-glutathione that occurs naturally in our organism composed of the three amino acids glutamic acid, L-cysteine and L-glycine important for the mitochondria, the "power plants of the cells", in which energy is produced in the form of ATP present in high amounts in the liver, kidneys, pancreas, spleen and eyes Possible causes for an increased L-glutathione requirement or deficiency: Lifestyle Stress High physical strain Low-protein diet Reduction diets Organism Intake of certain medications* Old age In case of diseases* Trauma Chronic fatigue syndrome Ulcerative colitis Cancer Sepsis * This refers to a possibly increased nutritional requirement and not to the alleviation, cure or prevention of a disease
After the lysis of the cells with RIPA lysis buffer, the cellular protein levels were measured using a bicinchoninic acid assay (Beyotime)
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