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Total RNA was extracted using the TRIzol reagent (Invitrogen, USA), quantified on an Agilent 2100 bioanalyser (Agilent Technologies, CA, USA) and a NanoDrop spectrophotometer (Thermo Fisher Scientific Inc.) and separated on 1% agarose gel

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Apart from lipid raft formation, reports also suggest that LPS-induced de novo lipogenesis is important for phagocytosis as it requires ongoing lipid synthesis in the ER for membrane expansion, such that Ms can surround and capture targeted pathogens for internalization (17, 18)

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