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The second step involves 3-hydroxy- N -trimethyllysine aldolase enzyme (HTMLA gene) along with vitamin B6 as a cofactor.[ref] Carnitine absorption and cellular transport: SLC22A5 Carnitine is transported into cells using the OCTN2 transporter or through passive transport

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In the quest for novel metabolic targets, approaches such as CRISPRCas9-mediated synthetic lethality screens targeting metabolic genes offer a pathway for identifying specific metabolic targets of interest, particularly in vivo