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For GSH assay, cells were treated in Earles balanced salt solution (EBSS), supplemented with 25 mM glucose, 4 mM glutamine, 1 X MEM vitamin solution (Life technologies, CA, USA), 0.22% wt/vol NaHCO 3 , 1 mM sodium pyruvate, 0.2% BSA, 25 mM HEPES, 100 U/ml penicillin and 100 g/ml streptomycin in the presence or absence of 1 X MEM amino acid solution (Gibco, CA, USA) with or without 100 M cystine

Possibly, the similar beneficial effects in other species (i.e., birds [296] and insects [297,298,299]) may suggest that BPC 157 may also have an extended regulatory physiologic role in bodily functions

The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and is easily adaptable for automation without a separation step

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Key differences: exogenous GH suppresses endogenous GH production through negative feedback (IGF-1 hypothalamus), while ipamorelin does not