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Sample lysates were separated via 414% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), following which the proteins were transferred to polyvinylidene difluoride (PVDF) membranes

The secretory IgA antibody (sIgA) is synthesized in the intestine in particular [8, 9, 16, 17]

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However, some preparations may have different timelines

Omega-3 fatty acids from fish oil provide natural anti-inflammatory support