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After electrophoresis, the gel was stained with a staining solution [0.1 % coomassie brilliant blue (CBB) R250, 45 % methanol, and 10 % acetic acid] for 2 h

The bilateral iliolumbar veins were then ligated with 70 sutures, along with the dorsal refluxing veins of the inferior vena cava and visualization of the refluxing inferior vena cava side branches

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2012 Apr;23(2):97-102