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For intracellular melanin quantification following MITF depletion, iMCs were transfected with 50 nM siMITF (ON-TARGETplus Mouse Mitf siRNA SMARTPool, L-047441-00-0005, Dharmacon, UK) or control siRNA (siGENOME Control Pool, D-001206-14-20, Dharmacon, UK) using the Viromer Blue kit (Lipocalyx) 4 h after plating