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Protein expression was induced with 0.5 mM isopropyl -D-1-thiogalactopyranoside (IPTG), and cultures were incubated overnight at 30 C

Avoid rubbing or pressing on the injection site for the first hour

Using the wrong concentration in your calculations can result in doses five to twenty times higher than intended

Prep Time : 3 mins | Total Time : 3 mins | Servings : 1 Ingredients : 1 scoop vanilla or unflavored protein powder 1 cup cold water 1 large handful fresh spinach 1 tablespoon chia seeds 45 ice cubes Instructions : Add all ingredients to a blender

The funders had no role in the conception, design, analyses, interpretation, manuscript drafting, or decision to submit the manuscript

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