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In some embodiments, the amount of thermoreversible polymer (e.g., pluronic F127) in any formulation described herein is about 10% of the total weight of the formulation

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RNA sequencing Sequencing libraries were generated using the NEBNext UltraTM RNA Library Prep Kit for Illumina following the manufacturers recommendations, and index codes were added to attribute sequences to each sample

In the meantime, other well-studied protocols already exist to target these same hallmarks of aging, ranging from pharmacological approaches like rapamycin and SGLT-2 inhibitors to lifestyle strategies such as exercise and caloric restriction

Common lab protocols involve reconstitution in bacteriostatic water and subcutaneous injection in animal models using calibrated syringes to achieve precise dosing