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Finally, the 3-Cy3 labelling reaction was performed overnight at 4C in 20 l containing 100 pmol unlabeled RNA, 0.5 mM ATP, 200 pmol pCp-Cy3 (Jena Bioscience), 10 U RiboLock RNase Inhibitor (Thermo Fisher Scientific), 1x Ligation Buffer and 10 U T4 RNA Ligase (Thermo Fisher Scientific)

The study included 31,609 patients, of whom 6,479 (20.49%) received SGLT2 inhibitor treatment, while 25,130 (70.50%) received DPP-4 inhibitor treatment

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