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doi: 10.1681/ASN.V133708
The inhibition of ABTS + formation by antioxidants in the samples were expressed as Trolox equivalents, determined at 740 mm using a calibration curve plotted with different amounts of Trolox (Sigma Aldrich) ( Glutathione (GSH) Levels The level of GSH was determined in samples of serum and peritoneal lavage fluid of septic mice at 12 and 24 h post CLP induction using Ellman's reagent ( In vitro Studies Peripheral Blood Mononuclear Cells (PBMC) Isolation and in vitro Stimulation PBMC of healthy volunteers who were abstainers of alcohol and tobacco (both sexes, ages 20 to 45 years) were isolated from blood using Ficoll (Sigma-Aldrich)
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Nevertheless, despite the tremendous success of TP, they present limitations for drug delivery due to skin layers, mainly stratum corneum (Figure 1), a layer of 1020 m of death tissue which restricts the permeability of most of the hydrophobic and hydrophilic drugs (Raju et al., 2018
Zorzano et al., 1996)
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