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Additional evidence suggesting ROS involvement in promotion of adipogenesis comes from antioxidant supplementation experiments where lower levels of ROS resulting from antioxidants contribute to adipose tissue dysfunction and IR [106]

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Baseline measurements were taken after an initial 5 min incubation with coelenterazine-h or NanoGlo-containing HBSS, after which cells were treated with either a vehicle (PBS) or the respective ligands