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The First-Strand cDNA Synthesis SuperMix Kit (TransGen Biotech, Beijing, China) was used to synthesize the first-strand complementary DNA (cDNA), following the manufacturers instructions
Chains with the highest affinity for a specific antigen, in this case TNF, are selected from this library, then recombined to produce a fully human, albeit non-native, antibody directed against TNF
Peer review Peer review information Communications Biology thanks the anonymous reviewers for their contribution to the peer review of this work
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Avoid placing it in the door, where temperatures fluctuate, or at the back where it might accidentally freeze
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