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Likewise, Ding et al

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FGF21 also stimulates adipose tissue uptake of fatty acids derived from lipoproteins (i.e., VLDL and chylomicron) (228), which together with increased glucose uptake functions to direct energy to adipose tissue and effectively redirect calories away from the liver

Cell pellets were resuspended in LDOX lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 0.01 mg ml 1 DNAseI) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator

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