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To verify the knockdown of ARL8B and TBC1D9B, cells were lysed in a buffer containing 1% Igepal, 1 M Tris pH 8, 150 mM NaCl, and protease inhibitors cComplete mini, along with phosphatase inhibitor cocktails 2 and 3

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The lower panel shows the SHAP summary plot for the impact of baseline variables in predicting the change in BMI in response to semaglutide

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