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In contrast, Ramesh et al

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Briefly, induction of the animals anaesthetization was carried out with 3% V/V isoflurane in 100% O 2 , and for maintenance, 1.5% V/V isoflurane in 100% O 2 was used through a nose cone

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In contrast, sedentary behavior has been associated with microbial alterations that may favor obesity-related metabolic dysfunction