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Within the body, hydrogen peroxide is tightly regulated by antioxidant enzymes such as catalase, glutathione peroxidase, and peroxiredoxins

Additionally, we and others have observed that other lipoyl adducts can be formed when lipid peroxidation products accumulate or following nitrosylation during macrophage activation 15,16,17,18

Microsomes were prepared by sonication of 10 8 cells, removal of debris by centrifugation at 10,000 g for 15 min, and subsequent centrifugation of the resulting supernatant at 100,000 g for 60 min

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Cells were spun at 4C for 20 min to pellet, and resuspended in lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH = 8)