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After adding three internal standards and 400 L cold methanol, tissues were homogenized via TissueLyser II (QIAGEN, USA) and centrifuged (10,000g, 15 min, 4C) to isolate the supernatant

It plays a key role in detoxification, neutralizing free radicals, and maintaining cellular balance

They work well because they directly help in raising blood levels

For immunohistochemistry, antigen retrieval was performed by boiling in 10 mmolL 1 sodium citrate (pH 6.0) for 5 min

If lyophilized, you need to reconstitute tirzepatide with bacteriostatic water before use