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Reactions were performed in 200 L of 100 mM Tris-HCl (pH 8.0) containing 0.25 M Cp1B, 10 mM ATP, 12 mM MgCl 2 , 300 M NADH, 500 M phosphoenolpyruvic acid (PEP), 41 units/mL pyruvate kinase (PK, Sigma), 59 units/mL lactate dehydrogenase (LDH, Sigma), 10 mM KCl with 1 mM acid donors and 5 mM l-Phe

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TB-500 is a synthetic peptide fragment corresponding to the active region of Thymosin Beta-4 (TB4) a 43-amino acid G-actin sequestering protein that is one of the most abundant intracellular peptides in mammalian cells