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Examples: Purifying Fully Synthetic Semaglutide of Various Purity Example 1: Crude Purity 59.65% Stage 1 : Ultisil XB-C8, 10 m, 120 Result: Purity: 94.56% Recovery: 80% Loading Capacity: 0.6% (v/v relative to stationary phase) Stage 2 : Xtimate C8, 10 m, 120 Result: Purity: 99.65% Recovery: 71% Loading Capacity: 0.56% (v/v relative to stationary phase) Example 2: Crude Purity 82.75% Packing Material : Ultisil XB-C8, 10 m, 120 Result: Purity: 99.15% Recovery: 71% Loading Capacity: 0.62% (v/v relative to stationary phase) Conclusion For a low-purity semaglutide, using Welch Ultisil XB-C8 (10 m, 120 ) and Xtimate C8 (10 m, 120 ) to perform a two-stage purification, the final purity is 99.65% with a total recovery rate of 56.8%

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