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Cells were preincubated in glucose-free or 10 mM glucose containing Hanks Balanced Salt Solution (HBSS) (1.3 mM CaCl 2 , 0.8 mM MgSO 4 , 5.4 mM KCl, 0.44 mM KH 2 PO 4 , 4.2 mM NaHCO 3 , 137 mM NaCl, 0.34 mM Na 2 HPO 4 pH 7.4) for 1 h at 37C and 5% (v/v) CO 2

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Collectively, our findings establish the 1,2-dithiolane architecture as a dual-mode molecular scaffoldthrough strategic incorporation of hydroxyl or amino moieties as cargoesthat achieves orthogonal responsiveness to distinct redox regulators (e.g., thiols vs