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Acta Neuropathol Commun 2015;3:31

Chang, H., et al

Vitamin B12 is involved in choline, fatty acid and amino acid metabolism and other important processes in animals

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Also, amounts vary depending on the antibody source, as follows: for each 200-500 g of cell or tissue lysate protein, we suggest adding 1-10 g of purified monoclonal or polyclonal antibody, 1-5 L of an unpurified polyclonal antiserum, 0.2-1 L of ascites fluid, or 20-100 L of hybridoma supernatant

After 5 minutes, the reaction was stopped by the addition of 100 l of MBB in MeOH (10 mM)