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Lactate further augments neutrophil functions, including chemotaxis, phagocytosis, oxidative burst and neutrophil extracellular trap formation, via energy provision and PI3K/Akt signaling, which may exacerbate tissue cell ferroptosis (105)

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McCartney-Francis et al [31] diluted the Polymorphprep 5:1 for rat PMNs and the use of a high concentration of EDTA (7.7 mM) may reflect the aggregation problems sometimes encountered with rat blood [32]