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As a third example, aspartic acid has a predominant negative net charge above pH~4.4, thus for anion exchange the pH elution gradient with a solvent comprising an organic modifier could begin above pH 4.4 to remove a truncated form missing aspartic acid and end the gradient below pH 4.4 thereby subsequently eluting the target GLP-1 moiety

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Similar patterns are observed with tirzepatide discontinuation

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