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The protocol for immobilization of GST-SPINK3 to glutathioneagarose beads was modified from previously reported protocols by using an alternative bifunctional cross-linker (dithiobis(succinimidyl propionate)) in a very simple procedure and by using simple buffers under physiological conditions

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To enhance the practical applicability of this framework, future research should also address the heterogeneity in dosing, treatment duration, and patient phenotypes observed across existing studies