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20 ng of cDNA was used per qPCR reaction using Powerup SYBR green master mix (Thermo Fisher Scientific) and gene specific primers according to manufacturers instructions on a Quantstudio 3 Real-Time PCR system (Thermo Fisher Scientific)

The D 2 , D 3 , and D 4 dopamine receptors are coupled to G i -type G-proteins and, therefore, receptor activation results in the inhibition of adenylate cyclase

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MEDVis physician-guided protocols are structured to align dosing progression with individual response rather than fixed timelines