Real-time polymerase chain reaction (PCR) was performed by using a kit (SYBR Premix EX Taq, Takara Bio Inc.) and the ABI PRISM 7300 real-time PCR system
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This happens when DNA repair is defective, for example in known diseases and DNA repair capacity varies between individuals, some being at higher risk for hyperparylation
BPC 157 was given per-orally, in drinking water (10 g/kg, 10 ng/kg, i.e., 0.16 g/ml, 0.16 ng/ml, and 12 ml/rat/day) till sacrifice, or alternatively, 10 g/kg and 10 ng/kg intraperitoneally