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Secondary antibodies were then used for detection as follows: AlexaFluor488 goat anti-rabbit (1:500, Invitrogen, A11008), AlexaFluor647 goat anti-rabbit (1:500, Invitrogen, A21244)

Ureidopeptide GLP-1 analogues with prolonged activity in vivo via signal bias and altered receptor trafficking

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The article says the gradual approach supports adherence and reduces gastrointestinal complications, and describes an initial period at the starting strength for tolerance assessment before any progression

doi:10.2337/diabetes.50.1.96 Granado M, Garca-Cceres C, Frago LM, Argente J, Chowen JA

Serious side effects and their symptoms can include the following: Dehydration (low fluid level), which can cause other problems such as low blood pressure