Thaw each aliquot only once Protect from light: Store in the original amber or opaque vial, or wrap in aluminum foil Keep the stopper sealed: Always replace the cap after each use to maintain sterility For comprehensive storage protocols across multiple compound classes, the complete peptide storage guide provides temperature charts and stability data for common research compounds
By conversion of choline to betaine by betaine aldehyde dehydrogenase and choline dehydrogenase [11], the three methyl groups of choline are readily available for three different conversions: (1) sarcosine into glycine by sarcosine dehydrogenase enzyme, (2) by using betaine from betaine-homocysteine methyltransferase as methyl donor and converting homocysteine into methionine, and (3) in conversion of dimethylglycine into sarcosine by dimethylglycine dehydrogenase enzyme
PLoS ONE 9 , e83828 (2014)
Malakorn S, Ouchi A, Hu CY, Sandhu L, Dasari A, You YN, Kopetz ES , Ellis LM, Chang GJ
MD Rodriguez-Arnao, Madrid
Kos I, Patterson MJ, Znaidi S, Kaloriti D, da Silva Dantas A, Herrero-de-Dios CM, et al