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Phenylephrine (PE), acetylcholine (ACh), L-NAME, hemoglobin and sodium nitroprusside (SNP) were purchased from Sigma Chemical Company (St
Refrigerate unopened powder at 3646F (28C), freeze it for long-term storage, and use a reconstituted vial within 28 days
coli Rosetta2 (DE3) cells (Novagen) transformed with the above plasmids were cultured as previously reported (Schenck et al., 2017)
Repeat cycles as needed
To me it sounds more like a bad side effect if anything
Gene-level counts were generated using HTSeq, and genes with fewer than 10 reads in any library were excluded from downstream analysis
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