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To prepare the NbLumSyt1-pH/nb-At647N complex, a 110-time molar excess of NbLumSyt1-pH was mixed with anti-GFP NbLumSyt1-At647N (Camelid sdAB from Synaptic Systems, N0301-At647N-S) at 4C for 16 hours, covered from light, in gentle rotation (20 rpm)

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