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Engineering: Cell free biosynthesis of glutathione with ATP-regenerating system - poultry waste upcycle Introduction: Glutathione (GSH) is a tripeptide consisting of glycine, L-glutamic acid and L- cysteine

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The cysteine moiety of GSH can be liberated via -glutamyl cycle in which exported GSH is cleaved sequentially by two exofacial enzymes, namely -glutamyl transpeptidase (GGT) and dipeptidase (DP) to release cysteine which is then imported into the cells 6

doi: 10.1016/j.hest.2020.04.003 84 HassamalS