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5- CTTGATGGCCGGGAGCTGCTTCTTC, 5-GGCCAGATGCGCCTGTCCAAGAAG and 5- GGCGAGGATCTCGTCGTGACCCATG for Parp1, yielding a 200 bp wild-type DNA fragment and a 700 bp mutant DNA fragment

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Unless stated otherwise, PSCs were preincubated in DMEM containing 10% FBS and 5.5 mM glucose for 24 h

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10.1038/nm.3761 61 FontanellaR