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The cells were cultured in Eagles minimum essential medium (ThermoFisher Scientific) or DMEM F-12 (ThermoFisher Scientific) respectively, supplemented with 10% Fetal Bovine Serum (Gibco) and 1% penicillinstreptomycin (ThermoFisher Scientific), in a humidified incubator maintaining 5% CO 2 at 37 C

Las personas que tienen un IMC de ms de 30 tienen una indicacin de tratamiento farmacolgico, adems de las pautas higinico-dietticas (alimentacin y actividad fsica)

Therapeutic intervention The patient was prescribed a 7:1 water extract of SZRT (500 mg/tablet) to be taken twice daily (a classical TEAM formula traditionally used for insomnia and irritability)