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IGF-1 enhances protein synthesis, promotes muscle growth, supports tissue repair, and improves bone density (3)

In the procedure, bovine insulin was used as an internal standard and added to blood samples, followed by protein precipitation using a mixture of acetonitrile and methanol (70:30,v: v)

Individuals with autoimmune conditions or immunosuppression should exercise caution, as IGF-1s immune-modulating effects could theoretically exacerbate existing conditions

Membranes were incubated with primary antibody overnight diluted in TBS-T/milk (or TBS-T/BSA for anti-phospho antibodies) at 4 C, washed in TBS-T and incubated for 1 hour at RT with the corresponding HRP-conjugated secondary antibody in TBS-T/milk

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