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Venkataraman T, Frieman MB
Before measurements, the hydrogel samples were first lyophilized and sprayed with gold
Further immunoblotting analysis of the nitration level of HGF was performed in the same manner using anti-nitroY198/Y250-HGF mAbs (1:2500 dilution,clones 3A11C6 and 6B82C3 raised in-house specifically to visualize nitroY198-HGF and nitroY250-HGF, respectively, see Elgaabari et al