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The NIDA Research Technology Branch provided active cannabis and placebo for this study

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One more wash 3 5 min in Tris-HCl 50 mM (pH=7.5) at RT 4 mL/well (12-well plate) was done before mounting the slices on gelatine coated slides (adding Prolong Gold antifade mounting medium, Invitrogen, P36930)

Weeks 1-4: Side effects are often the most noticeable as the body adjusts to the initial dose