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Given that cases are projected to increase worldwide over the coming decades especially in low- and middle-income countries there is an urgent need to develop and deploy effective treatments for the disease

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In plasma, a soluble form of DPP-IV with preserved catalytic activity can be detected (Zhong et al

Fortes GB, Alves LS, de Oliveira R, Dutra FF, Rodrigues D, Fernandez PL, et al

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Key points Subcutaneous injection is performed under the skin and does not penetrate muscular tissue.[7] Subcutaneous injection is a relatively simple, quick, and inexpensive procedure.[7] This route often causes minimal pain or discomfort to the mouse, can be easily observed and treatment can be applied if required.[4, 7] The most usual site for sc injection in mice is over the shoulders, into the loose skin over the upper back and neck (Figure 2), but other sites with loose folds of skin can also be used, for example over the flank.[1, 4, 7] Injection angle should be shallow to avoid causing unnecessary damage to surrounding tissues.[7] Route may cause pain if the administered substance is irritant or cytotoxic.[4] Key Points to Consider When Performing Subcutaneous Injections in Mice Subcutaneous administration of material often causes minimal pain or discomfort Subcutaneous Injection Technique in Mice Restrain the mouse but loosely enough to ensure the skin is mobile