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89 During pregnancy, melanocytes may be sensitive to elevated levels of and MSH, as well as oestrogen, progesterone and endorphins, which likely stimulate melanin production

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In addition, rabbit monoclonal anti-E cadherin (Abcam, Cambridge, UK), rabbit monoclonal anti-Rps20 (Abcam), anti-SLC10A2 rabbit polyclonal antibody (Bioss Antibodies, Woburn, MA, USA), and anti-rabbit immunoglobulin Ghorseradish peroxidase secondary antibody (Sigma-Aldrich) were purchased for western blotting