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After incubation at 37 C for 6 h, the medium was replaced with incubation medium (Williams Medium E without phenol red supplemented with Hepatocyte Maintenance Supplement Pack)

it is oil-soluble (unlike L-ascorbic acid) allowing deeper skin penetration before the enzyme activity in skin converts it to active L-ascorbic acid inside the cell

Collect the cell lysate and centrifuge at 13,200 g at 4 C for 15 min, and the supernatants were used for LCMS analysis after diluting with water to proper concentrations

[6] Gok, B., Budama-Kilinc, Y., & Kecel Gunduz, S

doi: 10.1016/j.pharmthera.2021.108023 10

10.1016/S1079-9796(03)00065-2 76