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Maiorano BA, Schinzari G, Ciardiello D, Rodriquenz MG, Cisternino A, Tortora G, et al
Following incubation, caspase 3 activity was measured using the fluorogenic substrate AC-DEVD-AMC as described before ( 2 , 1.2 mM EGTA, 1.5% Nonidet P40, 0.3% CHAPS, 30% sucrose, 150 M caspase 3 substrate DEVD-AMC, 30 mM DTT, and 3.0 mM PMSF was directly added to HepG2 cells
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If you experience side effects from the shot, adjusting the dosage can be effective at reducing the occurrence
