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Prior to any procedure, animals are anesthetized using a combination of xylazine (10 mg/kg), ketamine (40 mg/kg) and acepromazine (0.75 mg/kg) for up to an hour via the intramuscular route

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Results Inhibition of glutathione metabolism depletes neuronal glutamate and affects excitatory transmission To test the hypothesis that glutathione is a significant reservoir for glutamate, we treated neuronal cells with molecular inhibitors targeting enzymes of the glutathione metabolic cycle: L-2-imidazolidone-4-carboxylate (2I4C), acivicin, L-buthionine sulfoximine (BSO), and sulforaphane

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