The reaction was initiated by the addition of 190 l reaction buffer, adjusted to pH 7.4 by titration with K 2 HPO 4 (40 mM) and KH 2 PO 4 (10 mM) stock solutions, supplemented with NADH (100 M) and sodium pyruvate (600 M)
This editing alters a Ser codon to a Gly codon resulting in increased affinity of the AZIN1 protein for the antizymes, particularly the OAZ1 encoded antizyme
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