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This enzyme catalyzes the N-methylation of nicotinamide using S-adenosyl-L-methionine (SAM) as a methyl donor, effectively creating a "metabolic drain" that depletes both NAD+ precursors and cellular methylation capacity

5 Characterization of Lysine 48 mutants of GPX4 in cells and in vitro

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Oxidized glutathione (GSSG) has already donated its electrons and must be converted back to GSH by glutathione reductase before it can function as an antioxidant again