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Vitamin B12 Deficiency: Many researchers believe that the standard American tests that measure serum B12 levels are far too low

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As such, programming may need to begin with machine-based or seated exercises, with gradual progression as physical function improves

The (His) 6 -tag was removed by overnight thrombin cleavage, followed by polishing with size-exclusion chromatography (SEC) using a Superdex 75 HiLoad 16/600 column (Cytiva, MA, USA) equilibrated in 20 mM Tris-HCl pH 8.0, and 150 mM NaCl