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Cell lines Hepa1-6 (mouse hepatocarcinoma cell line) and HepG2 (human hepatocarcinoma cell line) were purchased from KAC and cultured in DMEM (Wako) containing 10% FBS (Biowest)

Prior to behavioral studies, a guide cannula was positioned in the right hemisphere and was fitted with a beveled tip to stop the penetration depth at 2.5 mm